| Chemical Biology of Secondary Metabolism
Resume

Joris Beld

Recent publications

03.10.2016

Evolution of fatty acid synthases

Fatty acid supplementation reveals differences in fatty acid metabolism. Published in Molecular Biosystems.

03.10.2016

Advanced mechanistic "crosslinking"

ACP-enoyl reductase interaction. Published in JACS.

JORIS BELD
Microbiology and Immunology
Drexel University
Philadelphia
United States of America
e-mail: jorisbeld at gmail.com or joris.beld at drexelmed.edu


Objective
To develop a leading graduate research program in chemical biology with a focus on expanding the understanding of primary and secondary metabolism.

Personal details
Titles:               Ph. D. in Sciences and M. Sc. in Chemical Engineering
Nationality:     Dutch

Education and Employment

Assistant Professor | Drexel University | 2015 - current
See research.

Postdoctoral Fellow | Michael D. Burkart laboratory | UC San Diego | 2009 - 2015
Involved in several projects amongst which: Selenium as an NMR probe for acyl carrier protei dynamics; Solvatochromic dyes as probes for domain-interactions in fatty acid biosynthesis; and Manipulating fatty acid biosynthesis in micro algae.
Ph.D. Degree in Chemistry | Donald Hilvert laboratory | ETH Zürich | 2004 - 2009
Dissertation title: Selenocysteine as a probe of oxidative protein folding.
M.S. Degree in Chemical Engineering| Dr. David Reinhoudt | University of Twente | 1999 - 2003
Master’s of Science degree in Chemical Engineering with Prof. David Reinhoudt, Supramolecular Chemistry and Technology, part of MESA+ Nanotechnology Institute, University of Twente, Enschede, The Netherlands. Thesis title: Anion sensing by fluorescence using self assembled monolayers on glass.
Apprenticeship | Dr. Peter Timmerman | Pepscan Systems B.V. | 2001 - 2002
Project title: Binding bodies.
B.S. Degree in Chemical Engineering | University of Twente | 1995 - 1997
1997-1995        Bachelor’s of Science degree in Chemical Engineering, University of Twente, Enschede, The Netherlands.

Teaching
2009                   Teaching Assistant General Chemistry, Organic Chemistry II, ETH Zurich
2006-2007        Teaching Assistant Biological Chemistry, ETH Zurich
2005-2007        Master’s student supervisor, ETH Zurich
2004-2006        Teaching Assistant Organic Chemistry laboratory course, ETH Zurich


 

Publications

•   Ma, A.T., Beld, J. and Brahamsha, B.,An Amoebal Grazer of Cyanobacteria Requires Cobalamin Produced by Heterotrophic Bacteria; Applied and Environmental Microbiology, 2017

•   Finzel, K., Beld, J., Burkart, M.D. and Charkoudian, L.,Utilizing Mechanistic Cross-Linking Technology To Study Protein–Protein Interactions: An Experiment Designed for an Undergraduate Biochemistry Lab; Journal of Chemical Education, 2017

•   Thiele, G.A.C.,Friedman, C.P., Tsai, K.J.S., Beld, J., Londergan, C.H. and Charkoudian, L..,Acyl Carrier Protein Cyanylation Delivers a Ketoacyl Synthase–Carrier Protein Cross-Link; Biochemistry, 2017

•   Sonnenschein, E.C., Pu, Y., Beld, J. and Burkart, M.D., Phosphopantetheinylation in the green microalgae Chlamydomonas reinhardtii; Journal of Applied Phycology, online (2016)

•   Beld, J., Abbriano, R., Hildebrand, M. and Burkart, M.D., Probing fatty acid biosynthesis using chemistry, in bacteria, cyanobacteria, green microalgae and diatoms; Molecular Biosystems, 10.1039/C5MB00804B (2016)

•   Uranga, C.C., Beld, J., Burkart, M.D., Mrse, A., Ghassemian, M.,  Córdova Guerrero, I. and Hernández Martínez, R. Fatty acid ester production in the trunk disease fungus Lasiodiplodia theobromae; Biochemical and Biophysical Research Communications, accepted online (2016)

•   Tallorin, L.T., Finzel, K., Beld, J., La Clair, J.J. and Burkart, M.D., Trapping of the Enoyl-Acyl Carrier Protein Reductase• Acyl Carrier Protein Interaction, Journal of the American Chemical Society, accepted online (2016

•   Cahill, J. F., Darlington, T. K., Fizgerald, C., Shoepp, N., Beld, J., Burkart, M. D. and Prather, K. A., Editors' Highlight Previous Article Next Article Articles ASAP Online Analysis of Single Cyanobacteria and Algae Cells under Nitrogen-Limited Conditions Using Aerosol Time-of-Flight Mass Spectrometry; Analytical Chemistry, 2015, ASAP

•   Ferreira-Camargo, L., Tran, M., Beld, J., Burkart, M.D. and Mayfield, S. P., Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae; AMB Express, 2015, online

•   Beld, J., Cang, H., Burkart, M.D., Visualizing the chain-flipping mechanism in fatty acid biosynthesis, Angewandte Chemie, 2014

•   Beld, J., Lee, D. J. and Burkart, M.D., Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering, Molecular Biosystems, 2015

•   Beld, J., Finzel, K. and Burkart, M.D., Versatility of acyl-ACP synthetases Chemistry & Biology, 2014, highlighted in preview


•   Beld, J., Sonnenschein, E.C., Vickery, R.V., Noel, J.P. and Burkart, M.D., The Phosphopantetheinyl Transferases: Catalysis of a Posttranslational Modification Crucial for Life, Natural Products Reports, 31, 61-108 (2014)
•   Bruegger, J., Caldara, G., Beld, J., Burkart, M.D. and Tsai, S.-C. Polyketide Synthase: Sequence, structure, function in Natural Products: Discourse, Diversity and Design, ISBN: 978-1-118-29806-0 (2014)
•   Bruegger, J., Haushalter, R., Vagstad, A., Beld, J., Shakya, G., Mih, N., Townsend, C.A., Burkart, M.D. and Tsai, S.-C. Probing the Selectivity and Protein-Protein Interactions of a Nonreducing Fungal Polyketide Synthase Using Mechanism-Based Crosslinkers. Chemistry & Biology 20, 1135-1146 (2013) see corrigendum
•   Beld, J., Blatti, J.L., Behnke, C., Mendez, M., and Burkart, M.D. Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions, Journal of Applied Phycology, 1-11 (2013)
•   Blatti, J.L., Beld, J., Behnke, C., Mendez, M., Mayfield, S.P. and Burkart, M.D., Manipulating fatty acid biosynthesis in microalgae through protein-protein interactions, PLoS One, 7, e42949 (2012)
•   Metanis, N., Foletti, C., Beld, J. and Hilvert, D. Rescue of kinetically trapped intermediates in oxidative protein folding by selenoglutathione, Israel Journal of Chemistry, 51, 953-959 (2011)
•   Metanis, N., Beld, J. and Hilvert, D. The chemistry of selenocysteine. Patai’s chemistry of functional groups, edited by Z. Rappoport (2011)
•   Beld, J. Woycechowsky, K. J., and Hilvert, D. Diselenides as universal oxidative folding catalysts of diverse proteins, Journal of Biotechnology, 150, 481-489 (2010)
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Small molecule diselenides catalyze oxidative protein folding in vivo, ACS Chemical Biology, 5, 177-182 (2009) cover art: “In contrast to disulfides, small amounts of exogenously added diselenide restore motility, and cells swim to the edge of soft agar plates.”
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Diselenide resins for oxidative protein folding, patent application EP09013216 (2009)
•   Coquiere, D., Bos, J., Beld, J. and Roelfes, G., Enantioselective artifical metalloenzymes based on a bPP Scaffold, Angewandte Chemie International Edition, 121, 6894-6897 (2009)
•   Beld, J., Woycechowsky, K. J., and Hilvert, D. Catalysis of oxidative protein folding by small-molecule diselenides, Biochemistry 47, 6985-6987 (2008)
•   Beld, J., Woycechowsky, K.J., and Hilvert, D. Selenocysteine as a probe of oxidative protein folding, Oxidative folding of Proteins and Peptides, edited by J. Buchner and L. Moroder, Royal Society of Chemistry (2008)
•   Steinmann, D., Nauser, T., Beld, J., Tanner, M., Günther, D., Bounds, P. L., Koppenol, W. H. Kinetics of tyrosyl radical reduction by selenocysteine, Biochemistry 47, 9602-9607 (2008)
•   Beld, J., Woycechowsky, K. J., and Hilvert, D. Selenoglutathione: Efficient oxidative protein folding by a diselenide, Biochemistry 46, 5382-5390 (2007)
•   Timmerman, P., Beld, J., Puijk, W. C., and Meloen, R. H. Rapid and quantitative cyclization of multiple peptide loops onto synthetic scaffolds for structural mimicry of protein surfaces, ChemBioChem 6, 821-824 (2005)
•   Basabe-Desmonts, L., Beld, J., Zimmerman, R. S., Hernando, J., Mela, P., García Parajo, M. F., Van Hulst, N. F., Van Den Berg, A., Reinhoudt, D. N., and Crego-Calama, M. A simple approach to sensor discovery and fabrication on self-assembled monolayers on glass, J. Am. Chem. Soc. 126, 7293-7299 (2004)
•   Timmerman, P., Beld, J., Meloen, R. H., and Puijk, W. C. Method for selecting a candidate drug compound, International patent WO-2004-077062 (2004)


Selected Presentations

Beld, J., Probing protein-protein interactions in fatty acid biosynthesis, Tianjin University (September 2016) oral presentation

Beld, J., Unnatural fatty acid supplementation reveals major differences in fatty acid metabolism, AOAIS 2016, Wuhan, China (September 2016) oral presentation

Wilson, Rachael; Ma, Amy and Beld, Joris; Isolation of Myxobacteria from local bark for the discovery of new antibiotics; poster presentation Discovery Day 2016

Beam, Gabrielle Rose; Ma, Amy and Beld, Joris; Fatty acid metabolism in Vibrio cholerae; poster presentation Discovery Day 2016

Wilson, Rachael; Ma, Amy and Beld, Joris; Isolation of Myxobacteria from local bark for the discovery of new antibiotics, poster presentation IMMID 2016

Beld, J. Visualization of chain flipping in acyl carrier proteins using solvatochromic dyes, Haverford College (April 2016), oral presentation

Beld, J. Versatility of acyl-acyl carrier protein synthetases, Haverford College (February 2016) oral presentation

Beld, J. The Acyl Carrier Protein: visualizing chain flipping using solvatochromic dyes, Biochemistry departmental seminar, Drexel University College of Medicine, Philadelphia (December 2015), oral presentation

Beld, J. Visualization of chain flipping using solvatochromic dyes, Chemistry Department departmental seminar, Drexel University (October 2015), oral presentation

Beld, J. How bacteria make complex molecules: understanding and engineering synthases, Microbiology and Immunology departmental seminar, Drexel University College of Medicine, Philadelphia (September 2015), oral presentation

Beld, J., Protein-protein interactions in fatty acid biosynthesis, NPAG 10, San Diego (April 2015), oral presentation

Beld, J. Revealing flexibility in fatty acid biosynthesis using acyl-acyl carrier protein synthetases, New Orleans, ASM (2015), poster presentation

Beld, J., Michaud, J., Pu, Y., Schoepp, N. and Burkart, M.D., Chemistry approaches to algal biotechnology: from natural production isolation to metabolic engineering, CALCAB FF21, San Diego (2015), poster presentation

Ferreira-Camargo, L., Tran, M., Tusakul, L., Beld, J., Burkart, M. D., Mayfield, S. P., Chemical and biological strategies for protein accumulation in Chlamydomonas reinhardtii chloroplasts, CALCAB Symposium, San Diego (January 2014), poster, award received

Beld, J., Interrogating the fatty acid synthase in bacteria and algae, Hilvert Group Reunion, Zurich, Switzerland (June 2014), oral presentation 3. Beld, J., Probes for fatty acid synthase dynamics, Natural Product Affinity Group (NPAG), San Diego, USA (February 2013), http://npag.ucsd.edu, oral presentation

Beld, J., Schoepp, N., Pu, Y. and Burkart, M. D., Algae research in the Burkart Lab: Metabolic food for thought, CALCAB Symposium, San Diego (January 2014), poster

Lee, D. J., Beld, J., Vuong, H., Vickery, C. and Burkart, M. D., The dynamic acyl carrier protein: chain length effects and protein-protein interactions, GRC Bioorganic, Proctor Academy (June 2014), poster presentation

Beld, J. Cang, H., Burkart, M. D., Visualizing the chain-flipping mechanism in fatty acid biosynthesis, GRC Bioorganic, Proctor Academy (June 2014), poster presentation

Beld, J., Interrogating the fatty acid synthase in bacteria and algae, NOBCCHe, San Diego, USA (March 2014), http://ucsd.academia.edu/NOBCChEUCSD, oral presentation

Beld, J., Fatty acid synthase evolution teaches us protein-protein interactions, San Diego Center for Algae Biotechnology (SDCAB), San Diego, USA (January 2013), http://algae.ucsd.edu/student-postdoc-symposiums.html, oral presentation

•   Beld, J. Probes for fatty acid synthase dynamics, Natural Product Affinity Group (NPAG), San Diego, USA (February 2013), http://npag.ucsd.edu, oral presentation
•   Beld, J. Fatty acid synthase evolution teaches us protein-protein interactions, San Diego Center for Algae Biotechnology (SDCAB), San Diego, USA (January 2013), http://algae.ucsd.edu/student-postdoc-symposiums.html, oral presentation
•   Beld, J. and Burkart, M.D., Tuning fatty acid biosynthesis in algae by redox chemistry, San Diego Center for Algae Biotechnology (SDCAB), San Diego, USA (March 2012), oral presentation
•   Beld, J., Woycechowsky, K.J, Hilvert D. Diselenides catalyze protein folding in vivo, Natural Product Affinity Group (NPAG), San Diego, USA (September 2010), oral presentation
•   Beld, J., Woycechowsky, K.J., Hilvert, D. Diselenides are oxidative protein folding catalysts in vivo, EUCHEM conference on stereochemistry - Bürgenstock, Brunnen, Switzerland (May 2009)
•   Beld, J., Woycechowsky, K.J., Hilvert, D. Catalysis of oxidative protein folding by diselenides, EMBL conference on Chemical Biology 2008, Heidelberg, Germany (October 2008)
•   Beld, J., Woycechowsky, K.J., Hilvert, D. Efficient oxidative protein folding by selenoglutathione, 1st Global Centre of Excellence Symposium on Bio-Environmental Chemistry, Osaka, Japan (January 2008) Poster presentation award received.
•   Beld, J., Woycechowsky, K.J., Hilvert, D. Selenoglutathione, ESF Research Conference on Probing the Molecular Basis of Protein Function through Chemistry, San Feliu de Guixols, Spain (October 2005)
Fellowships
•   Netherlands Organisation for Scientific Research (NWO) Rubicon Postdoctoral fellowship (2009-2011)
•   Swiss National Fund (SNF) Postdoctoral fellowship (2009) Declined funding due to concurrency of the NWO award.
Professional Skills
•   Cell culture
•   Gas chromatography and GCMS analysis of fatty acids and metabolites
•   Computational protein-protein and substrate-enzyme docking
•   Detailed (Baysian) phylogenetic tree analyses
•   Lipid and fatty acid assays (UV spectroscopy, fluorescence, lipid thin layer chromatography)
•   Beckman XL-A analytical ultracentrifugation for protein molecular weight, oligomerization state and protein-protein binding efficiency determination
•   Manual and automated solid phase peptide synthesis (ABI 433A)
•   Multistep organic synthesis (including selenium synthesis)
•   NMR spectroscopy (small molecule and protein)
•   Protein (sub)cloning, expression and purification
•   HPLC/LCMS analysis, repair and maintenance (Agilent, Waters and Thermo-Finnigan)
•   Linked/non-linked computer System Administration (Linux, Macintosh, Windows)
•   Behringer Simon lecture series coordination (http://www.behringersimonlecture.ethz.ch)
Languages
•   Fluent in Dutch, English and German
•   Read and speak French